CZI File Documentation

Overview

Feature Value
File Extension .czi
Developed by Carl Zeiss Microscopy GmbH
Format Type Image
MIME Type image/x-czi
Compression Supports both uncompressed and compressed (JPEG, JPEG XR) images
Image Type Multi-dimensional (2D, 3D, Time-lapse, etc.)
Metadata Support XML-based rich metadata about microscopy conditions
Color Space Grayscale, RGB, CYM, and others
Objective Information Included (magnification, numerical aperture, etc.)
Image dimensions Scalable from small to very large images
Multi-Channel Supports multiple channels for different stains or fluorescent tags
Pyramidal Layers Multi-resolution pyramidal levels for efficient viewing
Annotation Support Embedded annotations for tagging regions of interest
File Integrity Checksums for data integrity verification
Compatibility Can be opened with Zeiss software and several third-party tools
Acquisition Parameters Stores extensive acquisition settings (laser power, exposure time, etc.)
Scalability Efficient storage for scaling from small to large datasets
Light Path Information Details on light paths including filters and beam splitters
Sample Information Details about the sample including labels and preparation
Versioning Supports file versioning to track changes or updates